QUANTITATIVE DETERMINATION OF BERBERINE IN THE ABOVEGROUND ORGANS OF MAHONIA AQUIFOLIUM (PURSH) NUTT
Downloads
Our study's objective was to use high-performance liquid chromatography (HPLC) to quantify the amount of berberine in the total amount of alkaloids extracted from M. aquifolium. Eclipse plus C-18 was used to separate the crude extract (250 x 4.6 x 5 µm). Methanol (0.1% formic acid): water (0.1 formic acid) 35:65 v v-1 made up the mobile phase. At 346 nm, ultraviolet detection was carried out. With a r2 > 0.9998, a solid linear response was seen in the concentration range under study. The alkaloid extract from the aerial parts of M. aquifolium should contain at least 2% berberine, according to the results. With intra- and inter-day variances of less than 1.03%, the approach is accurate. Within the range under study, linearity, sensitivity, and precision are all adequate.
Downloads
Metrics
(1) Ferreira, M.-J. U. Alkaloids in Future Drug Discovery. Molecules 2022, 27 (4), 1347. https://doi.org/10.3390/molecules27041347.
(2) Martin, S. F. Bridging Known and Unknown Unknowns: From Natural Products and Their Mimics to Unmet Needs in Neuroscience. Accounts of Chemical Research 2022, 55 (17), 2397–2408. https://doi.org/10.1021/acs.accounts.1c00773.
(3) Wang, H.; Xu, X.; Wang, X.; Guo, W.; Jia, W.; Zhang, F. An Analytical Strategy for Discovering Structural Analogues of Alkaloids in Plant Food Using Characteristic Structural Fragments Extraction by High Resolution Orbitrap Mass Spectrometry. LWT 2022, 154, 112329. https://doi.org/10.1016/j.lwt.2021.112329.
(4) Singh, P. A.; Bajwa, N.; Chinnam, S.; Chandan, A.; Baldi, A. An Overview of Some Important Deliberations to Promote Medicinal Plants Cultivation. Journal of Applied Research on Medicinal and Aromatic Plants 2022, 100400. https://doi.org/10.1016/j.jarmap.2022.100400.
(5) Bhambhani, S.; Kondhare, K. R.; Giri, A. P. Diversity in Chemical Structures and Biological Properties of Plant Alkaloids. Molecules 2021, 26 (11), 3374. https://doi.org/10.3390/molecules26113374.
(6) Rajput, A.; Sharma, R.; Bharti, R. Pharmacological Activities and Toxicities of Alkaloids on Human Health. Materials Today: Proceedings 2022, 48, 1407–1415. https://doi.org/10.1016/j.matpr.2021.09.189.
(7) Matsuura, H. N.; Fett-Neto, A. G. Plant Alkaloids: Main Features, Toxicity, and Mechanisms of Action. Plant Toxins 2015, 1–15. https://doi.org/10.1007/978-94-007-6728-7_2-1.
(8) Heinrich, M.; Mah, J.; Amirkia, V. Alkaloids Used as Medicines: Structural Phytochemistry Meets Biodiversity—an Update and Forward Look. Molecules 2021, 26 (7), 1836. https://doi.org/10.3390/molecules26071836.
(9) Och, A.; Podgórski, R.; Nowak, R. Biological Activity of Berberine—a Summary Update. Toxins 2020, 12 (11), 713. https://doi.org/10.3390/toxins12110713.
(10) García-Muñoz, A. M.; Desirée Victoria-Montesinos; Ballester, P.; Begoña Cerdá; Zafrilla, P. A Descriptive Review of the Antioxidant Effects and Mechanisms of Action of Berberine and Silymarin. Molecules 2024, 29 (19), 4576–4576. https://doi.org/10.3390/molecules29194576.
(11) Naz, I.; Masoud, M. S.; Chauhdary, Z.; Shah, M. A.; Panichayupakaranant, P. Anti-Inflammatory Potential of Berberine-Rich Extract via Modulation of Inflammation Biomarkers. Journal of Food Biochemistry 2022, 46 (12), e14389. https://doi.org/10.1111/jfbc.14389.
(12) Cheng, Z.; Kang, C.; Che, S.; Su, J.; Sun, Q.; Ge, T.; Guo, Y.; Lv, J.; Sun, Z.; Yang, W.; Li, B.; Li, X.; Cui, R. Berberine: A Promising Treatment for Neurodegenerative Diseases. Frontiers in Pharmacology 2022, 13. https://doi.org/10.3389/fphar.2022.845591.
(13) Kang, H.-G.; Wei, M.-L.; Wang, J.-L.; Ma, C.-P.; Zhang, X.; Huang, B.-W.; Xin, L.-S.; Bai, C.-M. Berberine, a Natural Compound That Demonstrates Antiviral Effects against Ostreid Herpesvirus 1 Infection in Anadara Broughtonii. Viruses 2025, 17 (2), 282. https://doi.org/10.3390/v17020282.
(14) Sun, L.; Yu, Q.; Peng, F.; Sun, C.; Wang, D.; Pu, L.; Xiong, F.; Tian, Y.; Peng, C.; Zhou, Q. The Antibacterial Activity of Berberine against Cutibacterium Acnes: Its Therapeutic Potential in Inflammatory Acne. Frontiers in Microbiology 2024, 14. https://doi.org/10.3389/fmicb.2023.1276383.
(15) Xie, W.; Su, F.; Wang, G.; Peng, Z.; Xu, Y.; Zhang, Y.; Xu, N.; Hou, K.; Hu, Z.; Chen, Y.; Chen, R. Glucose-Lowering Effect of Berberine on Type 2 Diabetes: A Systematic Review and Meta-Analysis. Frontiers in Pharmacology 2022, 13, 1015045. https://doi.org/10.3389/fphar.2022.1015045.
(16) Li, D.-D.; Yu, P.; Xiao, W.; Wang, Z.-Z.; Zhao, L.-G. Berberine: A Promising Natural Isoquinoline Alkaloid for the Development of Hypolipidemic Drugs. Current Topics in Medicinal Chemistry 2020, 20 (28), 2634–2647. https://doi.org/10.2174/1568026620666200908165913.
(17) Gao, Y.; Nie, K.; Wang, H.; Dong, H.; Tang, Y. Research Progress on Antidepressant Effects and Mechanisms of Berberine. Frontiers in pharmacology 2024, 15. https://doi.org/10.3389/fphar.2024.1331440.
(18) Yu, M.; Jin, X.; Liang, C.; Bu, F.; Pan, D.; He, Q.; Ming, Y.; Little, P.; Du, H.; Liang, S.; Hu, R.; Li, C.; Hu, Y. J.; Cao, H.; Liu, J.; Fei, Y. Berberine for Diarrhea in Children and Adults: A Systematic Review and Meta-Analysis. Therapeutic Advances in Gastroenterology 2020, 13, 1756284820961299. https://doi.org/10.1177/1756284820961299.
(19) Almatroodi, S. A.; Alsahli, M. A.; Arshad Husain Rahmani. Berberine: An Important Emphasis on Its Anticancer Effects through Modulation of Various Cell Signaling Pathways. Molecules 2022, 27 (18), 5889–5889. https://doi.org/10.3390/molecules27185889.
(20) Liu, Y.; Nwafor, E.-O.; Li, Z.; Wang, J.; Liu, Z. Effects of Berberine on Liver Cancer. Natural Product Communications 2022, 17 (5), 1934578X2211020. https://doi.org/10.1177/1934578X221102032.
(21) Xiong, R.-G.; Huang, S.-Y.; Wu, S.-X.; Zhou, D.-D.; Yang, Z.-J.; Saimaiti, A.; Zhao, C.-N.; Shang, A.; Zhang, Y.-J.; Gan, R.-Y.; Li, H.-B. Anticancer Effects and Mechanisms of Berberine from Medicinal Herbs: An Update Review. Molecules (Basel, Switzerland) 2022, 27 (14), 4523. https://doi.org/10.3390/molecules27144523.
(22) Jiang, S.-X.; Qi, B.; Yao, W.-J.; Gu, C.-W.; Wei, X.-F.; Zhao, Y.; Liu, Y.-Z.; Zhao, B.-S. Berberine Displays Antitumor Activity in Esophageal Cancer Cells in Vitro. World Journal of Gastroenterology 2017, 23 (14), 2511–2511. https://doi.org/10.3748/wjg.v23.i14.2511.
(23) Yamashita, A.; Kasai, H.; Maekawa, S.; Tanaka, T.; Yasunori Akaike; Akihide Ryo; Enomoto, N.; Kohji Moriishi. Berberine Promotes K48-Linked Polyubiquitination of HNF4α, Leading to the Inhibition of HBV Replication. Antiviral Research 2024, 232, 106027–106027. https://doi.org/10.1016/j.antiviral.2024.106027.
(24) Liu, X.; Wang, L.; Tan, S.; Chen, Z.; Wu, B.; Wu, X. Therapeutic Effects of Berberine on Liver Fibrosis Are Associated with Lipid Metabolism and Intestinal Flora. Frontiers in Pharmacology 2022, 13. https://doi.org/10.3389/fphar.2022.814871.
(25) Wang, Q.; Shen, W.; Shao, W.; Hu, H. Berberine Alleviates Cholesterol and Bile Acid Metabolism Disorders Induced by High Cholesterol Diet in Mice. Biochemical and Biophysical Research Communications 2024, 719, 150088. https://doi.org/10.1016/j.bbrc.2024.150088.
(26) Bu, P.; Le, Y.; Zhang, Y.; Zhang, Y.; Cheng, X. Berberine-Induced Inactivation of Signal Transducer and Activator of Transcription 5 Signaling Promotes Male-Specific Expression of a Bile Acid Uptake Transporter. Journal of Biological Chemistry 2017, 292 (11), 4602–4613. https://doi.org/10.1074/jbc.M116.757567.
(27) Chen, X.-H.; Xiang, K.-L.; Lian, L.; Peng, H.-W.; Erst, A. S.; Xiang, X.-G.; Chen, Z.-D.; Wang, W. Biogeographic Diversification of Mahonia (Berberidaceae): Implications for the Origin and Evolution of East Asian Subtropical Evergreen Broadleaved Forests. Molecular Phylogenetics and Evolution 2020, 151, 106910. https://doi.org/10.1016/j.ympev.2020.106910.
(28) NIKA TODUA; СHINCHARADZE, D.; KETEVAN MCHEDLIDZE; JEMAL ANELI; VACHNADZE, N. STUDYING MAHONIA AQUIFOLIUM (PURSH) NUTT., INTRODUCED in GEORGIA, for the CONTENT of ALKALOIDS and the ESTABLISHMENT of MICROSTRUCTURAL DIAGNOSTIC SIGNS of the PLANT. EXPERIMENTAL & CLINICAL MEDICINE GEORGIA 2022. https://doi.org/10.52340/jecm.2022.06.13.
(29) He, J.-M.; Mu, Q. The Medicinal Uses of the Genus Mahonia in Traditional Chinese Medicine: An Ethnopharmacological, Phytochemical and Pharmacological Review. Journal of Ethnopharmacology 2015, 175, 668–683. https://doi.org/10.1016/j.jep.2015.09.013.
(30) The world flora online, https://www.worldfloraonline.org/search?query=Mahonia+aquifolium
(31) Xu, P.; Xu, C.; Li, X.; Li, D.; Li, Y.; Jiang, J.; Yang, P.; Duan, G. Rapid Identification of Berberine Metabolites in Rat Plasma by UHPLC-Q-TOF-MS. Molecules 2019, 24 (10), 1994. https://doi.org/10.3390/molecules24101994
Copyright (c) 2025 Georgian Scientists
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
