THE STUDY OF CYTOTOXIC ACTIVITY OF SOME ALKALOID-BEARING PLANTS GROWING IN GEORGIA

Lali Kintsurashvili; Tamar Suladze; Nino Kachlishvili; Jean Legault; Vakhtang Mshvildadze; Nina Vachnadze

doi:10.52340/gs.2026.08.01.14

Authors

Lali Kintsurashvili Tbilisi State Medical University
Tamar Suladze Tbilisi State Medical University
Nino Kachlishvili Tbilisi State Medical University
Jean Legault Université du Québec à Chicoutimi (Canada)
Vakhtang Mshvildadze Tbilisi State Medical University
Nina Vachnadze Tbilisi State Medical University

DOI:

https://doi.org/10.52340/gs.2026.08.01.14

Abstract

The aim of the study was to examine the aboveground parts of plants growing in Georgia: Delphinium elisabethae N. Busch., Solanum nigrum L., Sophora japonica L., Vinca herbacea Waldst. et Kit., for their alkaloid content and to evaluate their biological activity. Purified total alkaloid fractions were obtained using liquid-liquid extraction from the aforementioned plants, in the composition of which, as Phytochemical analysis revealed the major alkaloid constituents correspond to diterpene (D. elisabethae), steroidal (S. nigrum), quinolizidine (S. japonica), and indole-type bases (V. herbacea). Cytotoxic activity in vitro was assessed using three cell cultures: A-549 (human lung carcinoma cell culture, ATCC#CCL-185); DLD-1 (cell culture of the rectum adenocarcinoma, ATCC#CCL-221); WS-1 (human dermal fibroblasts) with the Resazurin and Hoechst tests.

It was found that the total alkaloid fraction from V. herbacea demonstrated strong cytotoxic activity against A-549 and DLD-1 cells, with no toxicity toward WS-1. The S. nigrum fraction displayed moderate cytotoxicity against all tested cell cultures. The total alkaloid fractions from D. elisabethae and S. japonica showed only weak cytotoxic activity against all cell lines.

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Author Biographies

Lali Kintsurashvili, Tbilisi State Medical University

TSMU, I. Kutateladze Institute of Pharmacochemistry, 36 P. Sarajishvili Street, 0159,Tbilisi, Georgia

Tamar Suladze, Tbilisi State Medical University

TSMU, I. Kutateladze Institute of Pharmacochemistry, 36 P. Sarajishvili Street, 0159,Tbilisi,

Georgia

Nino Kachlishvili, Tbilisi State Medical University

TSMU, I. Kutateladze Institute of Pharmacochemistry, 36 P. Sarajishvili Street, 0159,Tbilisi,

Georgia

Jean Legault, Université du Québec à Chicoutimi (Canada)

Université du Québec à Chicoutimi (Canada)

Vakhtang Mshvildadze, Tbilisi State Medical University

TSMU, I. Kutateladze Institute of Pharmacochemistry, 36 P. Sarajishvili Street, 0159,Tbilisi, Georgia

Université du Québec à Chicoutimi (Canada)

Nina Vachnadze, Tbilisi State Medical University

TSMU, I. Kutateladze Institute of Pharmacochemistry, 36 P. Sarajishvili Street, 0159,Tbilisi,

Georgia

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