4.1 Medicine 

Characteristics, Antibiotic and Phage Susceptibility of Staphylococci Causing Mastitis

resistance isolate strain bacteriophage antibiotic identification pathogen

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July 21, 2025

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The aim of the work is to isolate the pathogen based on the polyetiology of mastitis and the predominant role of staphylococci in the pathogenesis of the disease; to differentiate them by morphological, cultural and biochemical properties, and to study antibiotic and phage sensitivity. 13 strains of staphylococci were isolated and identified from milk samples. Among them, 8 St. aureus and 5 St. epidermidis field isolates. Their high sensitivity to doxycycline was revealed by the disk-diffusion method, the qualitative index of which was S. In addition, the polyresistance of some strains to azithromycin and streptomycin was determined to be 38.5-38.5%, and to amoxicillin to be 23.10%.

In the process of research, the complete lability of all staphylococcal strains to phage (100%) was revealed. Only 7.7% of staphylococci were found to be resistant to septaphage. The results obtained demonstrate the high lytic activity of phages and the prospect of using them as a drug devoid of side effects for the treatment of animals suffering from mastitis. A significant advantage of bacteriophage over other drugs is that bacteria do not have the property of developing resistance to it.

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