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Abstract
Sensitive, accurate and efficient Liquid Chromatographic Method is developed for the quantitative determination of Nitrofuran residues in fish samples. It was found that the coefficient of variation for all four test substances of the Nitrofuran group is from 0.03 ìg / ml less than 2%, respectively the detection minimum was considered to be 0.03ìg / ml; 376 nm wavelength is specific for Nitrofurans; For the quantification of nitrofuran residues in a fish sample validation of the developed Liquid Chromatographic Method revealed complete compliance of the method (Guidance for Industry Bioanalytical Method Validation U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) Center for Veterinary Medicine (CVM) May 2001) with the following validation characteristics of the requirements: detection minimum, specificity, accuracy, correctness and linearity.
References
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