How to Cite
Abstract
Taking into account the physico-chemical properties, the nature of the sorbent and the matrix, for solid-phase purification of extracted for meat nitrofuran metabolites we have proposed Sorbent - silica gel 60, particle size 0.2-0.5 mm, column size 20x40 mm, eluentethyl acetate, eluent volume 5 ml, sample and eluent speed 5 ml/min. The suitability of the proposed column has been confirmed experimentally by method validation. After purifying the nitrofurans extracted from the analyte sample with our proposed column, a recovery higher than 90% is achieved with good reproducibility (RSD ≤ 2.0%). The proposed column is specific, selective especially with respect to nitrofuran metabolites, increases the limit of quantitative determination of the HPLC method, which makes it possible to use a diode detector instead of expensive columns and a mass detector and, at relatively low financial costs, carry out the quantitative determination of nitrofuran metabolites within the limit established by the European Union (1 µg/kg). The proposed conditions for determining nitrofuran metabolites are relatively accessible and will allow them to be implemented by small farmers, developing countries and regions, which in turn ensures the safe use of meat and meat products.