DEVELOPMENT OF EXPERIMENTAL MODEL OF ACUTE HEPATITIS IN MICE USING SOLUTION OF ACETAMINOPHEN FOR INJECTION

Authors

  • Ghonghadze M. ღონღაძე მ.
  • Antelava N. ანთელავა ნ.
  • Okujava M. ოკუჯავა მ.
  • Pachkoria K. პაჭკორია ქ.

DOI:

https://doi.org/10.52340/csw.2015.49.0.131-133

Abstract

The goal of the research was to define the dose of acetaminophen’s  solution for injection needed to develop the model of hepatitis in mice. Initialy  the preliminary testing was provided and the dose, which caused the lethality in the part of experimental animals was found. Intraperitoneal injection of 500 mg/kg acetaminophen did not caused the death in mice, the lethality after injection of 750 mg/kg was 33% and  use of 1000 mg/kg drug was the reason of lethal outcome in 100%. We choose intraperitoneal injection of 750 mg/kg of acetaminophen for induction of hepatitis. After identification of the toxic dose we learned its effect on the weight of the liver and biochemical indexes. Injection of the toxic dose of acetaminophen significantly increased the weight of the liver  (47%) and the biochemical markers indicating hepatocyte cytolyses  (ALT – 88%, AST – 91%), thus the experimental model of hepatitis was developed.  Based on the obtained data we can conclude, that intraperitoneal injection of 750 mg/kg solution of acetaminophen is recommended to create the experimental model of acute hepatitis in mice, which is useful for investigation of hepatoprotective activity of different drugs. 

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

1. Буеверов А.О.. Патогенетические подходы к лечению лекарственных поражений печени. Гастроэнтерология .Заболевания печени 2008. №1, с. 17-23
2. Косенко И.М. Муколитические препараты в современной клинической практике Cons. Med. Педиатрия №1 / 2010 с. 52-59 -2
3. Скрыпник И.Н. Медикаментозные гепатиты: современные аспекты диагностики и лечения. Cons. Med. 2008. - Т. 10, № 8. - С. 48-52.
4. Соколовская А.Н.” Влияние лохеина на ме¬таболизм печени при острой интоксикации, выз¬ванной парацетамолом и D-галактозамином”, Бю¬ллетень сибирской медицины №3, 2006, с.48-52
5. Суханов Д. С., Винаградова Т. И., Заболотных Н. В., Коваленко А. Л., Васильева С. Н., Романцов М. Г., Сравнительное изучение гепатопро- тективного действия ремаксола, реамберина и адеметионина при повреждении печени про-тивотуберкулёзными препаратами, Антибитики и Химиотерапия, 2011; 56; 1-2; с. 12-16.
6. Aashish Pandit, Tarun Sachdeva and Pallavi Bafna Drug-Induced Hepatotoxicity: A Review Journal of Applied Pharmaceutical Science 02 (05); 2012: 233-243
7. Al-Majed A., Sayed-Ahmed M., Al-Omar F. et al. Carnitine esters prevent oxidative stress damage and energy depletion following transient forebrain ischaemia in the rat hippocampus. Clin Exp Pharmacol Physiol. 2006; 33(8): 725-733.
8. Bessone Fernando Non-steroidal anti-inflammatory drugs: What is the actual risk of liver damage? World J Gastroenterol. 2010 December 7; 16(45): 5651–5661.
9. Hinson Jack A., Roberts Dean W., and James Laura P. Mechanisms of Acetaminophen-Induced Liver Necrosis Handb Exp Pharmacol. 2010; (196): 369–405.
10. Karima Begriche, Julie Massart, Marie-Anne Robin, Annie Borgne-Sanchez Drug-induced toxicity on mitochondria and lipid metabolism: Mechanistic diversity and deleterious consequences for the liver. Journal of Hepatology Volume 54, Issue 4 , Pages 773-794, April 2011
11. Mitchell R. McGill, Matthew R. Sharpe, C. David Williams, Mohammad Taha, Steven C. Curry and Hartmut Jaeschke The mechanism underlying acetaminophen-induced hepatotoxicity in humans and mice involves mitochondrial damage and nuclear DNA fragmentation The Journal of Clinical Investigation, 2012;122(4):1574–1583.

Downloads

Published

2019-11-04

How to Cite

ღონღაძე მ. G. M., ანთელავა ნ. A. N., ოკუჯავა მ. O. M., & პაჭკორია ქ. P. K. (2019). DEVELOPMENT OF EXPERIMENTAL MODEL OF ACUTE HEPATITIS IN MICE USING SOLUTION OF ACETAMINOPHEN FOR INJECTION. TSMU COLLECTION OF SCIENTIFIC WORKS, 49, 131–133. https://doi.org/10.52340/csw.2015.49.0.131-133

Most read articles by the same author(s)