β-ADRENERGIC RECEPTOR BLOCKERS AS A REGULATOR OF T CELL VIABILITY (IN THE MODEL SYSTEM OF THE JURKAT CELLS)
DOI:
https://doi.org/10.52340/jecm.2022.06.002Ключевые слова:
β-blockers, T cells viability, adrenoreceptorsАннотация
Our research aimed to establish the effectiveness of various β-adrenergic receptor blockers in the regulation of T cell proliferation in the model system of the Jurkat cells.Material and Methods. The research was conducted on the human leukemic mature T cells (Jurkat cells) (DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen (Germany)) activated by phytohemagglutinin (PHA). Viability and proliferative activity of intact and PHA-stimulated Jurkat cells under the influence of Nebilet, Egilok, Betalok Zok, and Propranolol were studied with the MTT test. Results. Study results show that β-adrenergic receptors blockers selectively influence the activity of mitochondrial dehydrogenases, and therefore viability in both intact and mitogen-stimulated Jurkat cells. In particular, Nebilet, Betalok Zok, and Propranolol induced a statistically significant decrease of intact Jurkat cells viability by 63%, 20%, and 32%, respectively; Egilok and Betalok Zok (25mg) didn’t statistically significantly affect the PHA- activated Jurkat cells viability. Nebilet, Betalok Zok (50 mg), and Propranolol decreased the viability of the mitogen (PHA) activated Jurkat cells (for PHA dose 20μg/ml) and didn’t affect at a PHA dose of 50μg/ml.
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