COMPARABLE EFFECTIVENESS OF DIFFERENT ANTIHYPERTENSIVE AGENTS IN THE TREATMENT OF EXPERIMENTAL HYPERTENSION IN RATS

KETEVAN  KAKABADZE; IRAKLI  MEGRELADZE; NINO  KIPIANI; TAMAR  SANIKIDZE

doi:10.52340/jecm.2022.06.013

COMPARABLE EFFECTIVENESS OF DIFFERENT ANTIHYPERTENSIVE AGENTS IN THE TREATMENT OF EXPERIMENTAL HYPERTENSION IN RATS

Authors

KETEVAN KAKABADZE
IRAKLI MEGRELADZE
NINO KIPIANI
TAMAR SANIKIDZE

DOI:

https://doi.org/10.52340/jecm.2022.06.013

Keywords:

hypertension, nitric oxide, antihypertensive drug

Abstract

The aetiology and relationship between the NO content and the mechanisms of development of hypertension, whether dysfunction of the endothelium and a decrease in NO production have occurred before or after the development of hypertension, remains unclear. Arterial hypertension was induced in male Wistar rats (weighing 200-220 grams) by intraperitoneal administration of L-NAME (SIGMA-ALDRICH) for 4 and 7 weeks. During the last 3 weeks, to a separate group of animals simultaneously with L-NAME, L-arginine (SIGMA-ALDRICH) and different antihypertensive drugs (Nebilet+ (Nebivolol (beta-blocker) + hydrochlorothiazide), Lozap + (Specific antagonist of angiotensin II receptors), Hydrochlorothiazide (diuretic medication) and a combination of Norvasc (calcium channel blocker) + Hydrochlorothiazide) were administered. The animals were monitored for systolic and diastolic pressure. The level of NO in blood samples was determined spectrophotometrically using a Griess Reagent. The results of the study show that hypertension pathogenesis is very complex and includes a series of factors, additionally to the lack of vasodilatation compounds. Therefore, the effects of antihypertensive drugs are related to various mechanisms and require the presence of not only an additional substrate necessary for the synthesis of NO. Studying these mechanisms will help us to develop effective methods of treating hypertension.

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