INVESTIGATION OF EFFICACY OF ANTI-TUMOR PREVENTIVE VACCINATIONS WITH THE USE OF BACTERIAL PHAGELYSATES IN EXPERIMENTAL STUDIES

Abstract

We were aimed to investigate anti-tumor preventive potential of E. coli pagelysate vaccinations in experiment. Investigations were carried out on 2-3 months 48 male mice. Regimen of preventive vaccinations were: single - 0,25 ml E.coli phagelysate intraperitoneal injection, 3 days before Ehrlich carcinoma inoculation (1x10⁶ tumor cells), and 3 times vaccinations - 0,25 ml E.coli phagelysate injections with 5 day intervals, 3, 6, and 9 days before inoculation of carcinoma. Treatment efficacy was evaluated according to the indices of cancer growth (development of cancer tissue, cancer growth inhibition percent, lifespan and survival percent). Results were analyzed statistically with the use of SPSS 16.0 for Windows.  Experiments have shown that single and 3 times preventive E.coli phagelysate vaccinations are well tolerated in mice and do not stimulate malignant growth. 

Vaccinations inhibited tumor development and delayed the malignant growth. Cancer growth inhibition percent in vaccinated animals were 55% on the average. Maximal lifespan in control group  mice consisted 59 days.  By the 125th day of cancer growth, at single preventive vaccination, the 17% of mice were alive, while in 3 times vaccinated mice, the survival percent was 25%. Anti-tumor potential of E.coli pagelysate supposedly could be explained by immunoregulatory properties of preparation.