COMPOSITION AND TECHNOLOGY OF NATURAL AND MODIFIED BROMELAIN GELS

TAMAR  KORINTELI; NANA  GORGASLIDZE; ALIOSHA  BAKURIDZE; LIANA  NADIRASHVILI; GIORGI  ERKOMAISHVILI

doi:10.52340/jecm.2022.06.001

COMPOSITION AND TECHNOLOGY OF NATURAL AND MODIFIED BROMELAIN GELS

Authors

TAMAR KORINTELI
NANA GORGASLIDZE
ALIOSHA BAKURIDZE
LIANA NADIRASHVILI
GIORGI ERKOMAISHVILI

DOI:

https://doi.org/10.52340/jecm.2022.06.001

Keywords:

Bromelain, modified bromelain, gels, polyethylene, glycol

Abstract

Musculoskeletal disorders (MSDs) are one of the most pressing medical issues we face today, due to the modern, sedentary lifestyle of humankind. Because of the serious side effects of nonsteroidal anti-inflammatory drugs, searching for other remedies and selecting the best medicinal form of the drug still remains one of the major challenges in medicine.The aim of this research was the development of a gel for the treatment of Musculoskeletal pathologies. The proteolytic enzyme bromelain from the stems of the pineapple plant (Ananas comosus) was selected as the main active substance, due to its strong anti-inflammatory and analgesic properties. Its chemical modification with dextran aldehyde was performed to reduce its allergic effect and to increase stability. Statistical characteristics of the method for determining the proteolytic activity of bromelain were processed. A mixture of polyethylene glycol-1500 and polyethylene glycol-4000 was selected as the base of the gel. Stem bromelain and modified bromelain gels were prepared. The proteolytic activities of the developed gels were determined for 6 months every 3 months. The results demonstrated that proteolytic activities were maintained over the studied period of time, indicating that the gels are stable.

Downloads

Download data is not yet available.

References

Musculoskeletal conditions [Internet]. [cited 2022 Jun 1]. Available from: https://www.who.int/news-room/fact-sheets/detail/musculoskeletal-conditions

Pelletier JP, Martel-Pelletier J, Rannou F, Cooper C. Efficacy and safety of oral NSAIDs and analgesics in the management of osteoarthritis: Evidence from real-life setting trials and surveys. Seminars in Arthritis and Rheumatism. 2016 Feb 1;45(4, Supplement):S22–7.

Teslim O, Afolabi J. Side Effects of Non-Steroidal Anti-Inflammatory Drugs: The Experience of Patients with Musculoskeletal Disorders. American Journal of Health Research. 2014 Jan 1;2:106.

Gamarra F, Santana J, Vasquez Llanos S, Pérez J, Flausino F, Quispe A, et al. High Retention and Purification of Bromelain Enzyme (Ananas comosus L. Merrill) from Pineapple Juice Using Plain and Hollow Polymeric Membranes Techniques. Polymers. 2022 Jan 10;14:264.

Rahman MA, Imran TB, Islam S. Antioxidative, antimicrobial and cytotoxic effects of the phenolics of Leea indica leaf extract. Saudi J Biol Sci. 2013 Jul;20(3):213–25.

Rahaman MdM, Rakib A, Mitra S, Tareq AM, Emran TB, Shahid-Ud-Daula AFM, et al. The Genus Curcuma and Inflammation: Overview of the Pharmacological Perspectives. Plants (Basel). 2020 Dec 30;10(1):63.

Nwagu TN, Ugwuodo CJ. Stabilizing bromelain for therapeutic applications by adsorption immobilization on spores of probiotic Bacillus. Int J Biol Macromol. 2019 Apr 15;127:406–14.

Xue Y, Wu CY, Branford-White CJ, Ning X, Nie HL, Zhu LM. Chemical modification of stem bromelain with anhydride groups to enhance its stability and catalytic activity. Journal of Molecular Catalysis B, Enzymatic. 2010;3–4(63):188–93.

Brito AMM, Oliveira V, Icimoto MY, Nantes-Cardoso IL. Collagenase Activity of Bromelain Immobilized at Gold Nanoparticle Interfaces for Therapeutic Applications. Pharmaceutics. 2021 Jul 27;13(8):1143.

Shingade GM. REVIEW ON: RECENT TREND ON TRANSDERMAL DRUG DELIVERY SYSTEM. Journal of Drug Delivery and Therapeutics [Internet]. 2012 Jan 19 [cited 2022 Jun 1];2(1). Available from: https://jddtonline.info/index.php/jddt/article/view/74

Jeong WY, Kwon M, Choi HE, Kim KS. Recent advances in transdermal drug delivery systems: a review. Biomaterials Research. 2021 Jul 28;25(1):24.

Rathod H, Mehta D. A Review on Pharmaceutical Gel. International Journal of Pharmaceutical Sciences. 2015 Oct 1;1:33–47.

B SA, Jeganath S, Jeevitha E. Pharmaceutical Gels and Recent Trends –A Review. Research Journal of Pharmacy and Technology. 2019 Dec 30;12(12):6181–6.

Sopyan I, Intani W, Kurniawansyah I, S C. The Advantages of in situ Gel from Every Different Formulation. International Journal of Research in Pharmaceutical Sciences. 2020 Dec 24;11:7198–206.

Patil B, Akarte A, Chaudhari P, Wagh K, Patil P. Development and characteristics of topical gel containing nimesulide: A review. GSC Biological and Pharmac. Sciences. 2021 Jun 30;15:295–301.

Peppas NA, Bures P, Leobandung W, Ichikawa H. Hydrogels in pharmaceutical formulations. European Journal of Pharmaceutics and Biopharmaceutics. 2000 Jul 3;50(1):27–46.

Jang HJ, Shin CY, Kim KB. Safety Evaluation of Polyethylene Glycol (PEG) Compounds for Cosmetic Use. Toxicol Res. 2015 Jun;31(2):105–36.

ქიმიური ექსპერიმენტების შედეგების სტატისტიკური დამუშავება. - სახელმწიფო ფარმაკოპეა, 1998,1: 301-335.