IMPACT OF PROLONGED GLYCATION PRE-TREATMENT ON THE ELECTRON TRANSFER KINETICS OF CYTOCHROME C

TINATIN DOLIDZE; MAIA MAKHARADZE; SOPHIO UCHANEISHVILI; DIMITRI KHOSHTARIYA

doi:10.52340/jecm.2024.02.02

IMPACT OF PROLONGED GLYCATION PRE-TREATMENT ON THE ELECTRON TRANSFER KINETICS OF CYTOCHROME C

Authors

TINATIN DOLIDZE I. Beritashvili Center of Experimental Biomedicine
MAIA MAKHARADZE I. Beritashvili Center of Experimental Biomedicine
SOPHIO UCHANEISHVILI I. Beritashvili Center of Experimental Biomedicine
DIMITRI KHOSHTARIYA I. Beritashvili Center of Experimental Biomedicine

DOI:

https://doi.org/10.52340/jecm.2024.02.02

Keywords:

Cytochrome C, Glycation, D-glucose, cyclic voltammetry, electron transfer

Abstract

The effect of long term (up to 25 days) glycation process by D-glucose on the electron transfer properties of Cyt C under artificial conditions, using modified gold electrode, as a redox partner, has been studied. The redox kinetics of Cyt C, in the 0.1 M buffer solution containing 4 mg/mL Cyt C and 200 G/L D-glucose (T=25 ⁰C), by the glycation time more than three weeks was directly measured using the method of cyclic voltammetry. Systems voltamperic response has shown, that redox activity of glycated Cyt C is preserved upon the glycation time, at least, during the incubation period up to 25 days.

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