CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF AERIAL PARTS OF SALVIA SCLAREA L. CULTIVATED IN GEORGIA

TEONA KORKOTADZE; DALI BERASHVILI; MALKHAZ JOKHADZE; SOPIO GOKADZE; KETEVAN MCHEDLIDZE; VAKHTANG MSHVILDADZE

doi:10.52340/jecm.2023.04.19

CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF AERIAL PARTS OF SALVIA SCLAREA L. CULTIVATED IN GEORGIA

Авторы

TEONA KORKOTADZE Tbilisi State Medical University
DALI BERASHVILI Tbilisi State Medical University
MALKHAZ JOKHADZE Tbilisi State Medical University
SOPIO GOKADZE Tbilisi State Medical University
KETEVAN MCHEDLIDZE Iovel Kutateladze Institute of Pharmacochemistry
VAKHTANG MSHVILDADZE Iovel Kutateladze Institute of Pharmacochemistry

DOI:

https://doi.org/10.52340/jecm.2023.04.19

Ключевые слова:

Salvia sclarea L., Labiatae, phenolic compounds, biological activity

Аннотация

A study on the chemical composition of the essential oil obtained from the aerial parts of Salvia sclarea L., cultivated in Georgia, was carried out. The percentage of dominant components was determined by GC integration, the dominant components in the essential oil are linalool (26.81%) and linalyl acetate (42.99%). Quantitative content of dominant compound linalyl acetate, in Salvia sclarea essential oil, was also determined using a standard sample. From terpene family, oxygenated monoterpens are dominants. The content of phenolic compounds in the aqueous, methanolic and chloroformic fractions of the residual plant material after hydrodistilation, was determined by using Folin-Ciocalteu test. In aquaeous fraction total phenolic content was 13 ± 1%, in the methanolic fraction - 9.2 ± 0.9% and 11 ± 1% in the chloroformic fraction.

The essential oil has exhibited significant antioxidant activity in ORAC test as well as an antibacterial activity (E. coli, Salmonella enterica, Enterococcus faecium, Staphylococcus epidermidis).

Salvia sclarea essential oil, methanolic and chlorophormic fractions exhibited high anti-inflammatory activity with 85%, 92% and 96% inhibition of NO production respectively, at 77 μg/ml, 14 μg/ml and 13 μg/ml dose, without any significant toxicity. Methanolic fraction revealed a moderate cytotoxic activity (73 ± 4 μg/ml) against A-549 cell line.

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