DETERMINATION OF PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF GEORGIAN GRAPEVINE LEAVES

MARIAM TATANASHVILI; TAMAZ MURTAZASHVILI; MALKHAZ JOKHADZE; PAATA TUSHURASHVILI; KOBA SIVSIVADZE; NATIA BOKUCHAVA; SOFIO GOKADZE

doi:10.52340/jecm.2023.04.39

DETERMINATION OF PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF GEORGIAN GRAPEVINE LEAVES

Authors

MARIAM TATANASHVILI Tbilisi State Medical University
TAMAZ MURTAZASHVILI Tbilisi State Medical University
MALKHAZ JOKHADZE Tbilisi State Medical University
PAATA TUSHURASHVILI Tbilisi State Medical University
KOBA SIVSIVADZE Tbilisi State Medical University
NATIA BOKUCHAVA Tbilisi State Medical University
SOFIO GOKADZE Tbilisi State Medical University

DOI:

https://doi.org/10.52340/jecm.2023.04.39

Keywords:

Grapevine leaves, Phenolic compounds, Antioxidant activity

Abstract

SUMMARY

Vine products (wine, grapes) are rich in beneficial compounds for humans. However, Grapevine cultivation and grape processing generate a large number of by-products, such as grape seed, grape skin and grapevine leaf, most of them can equal or even exceed wine and grapes in terms of the content of biologically active compounds.

Grapevine leaves are a by-product of the vineyard maintenance process. According to the scant information that is currently accessible about Grape leaves, they are rich in organic acids, tannins, procyanidins, anthocyanins, enzymes, vitamins, and carotenoids.

From a scientific and practical standpoint, we considered it necessary to conduct a phytochemical analysis of grapevine leaves. The qualitative composition of phenolic compounds in Rkatsiteli and Saperavi grapevine leaves was examined using the high-performance liquid chromatography-tandem mass spectrometric method (LC-MS-MS) as a result of the performed studies. The obtained results indicate that grape leaves are particularly rich in phenolic acids and flavonoids. The following phenolic compounds have been identified: catechin, epicatechin, quercetin-3-0-glucoside, caffeic acid, trans-resveratrol, etc. (a total of 19 phenolic compounds). The antioxidant activity of the extracts prepared from the raw materials was also studied, which was determined using the DPPH reagent. During the research, a correlation was discovered between the phenolic content of the raw materials and their antioxidant activity. The findings indicate that grapevine leaves contain natural antioxidants that can be used in pharmaceutical, food, and cosmetic production.

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