ALTERATIONS OF ANTIOXIDANT ENZYMES DURING ALLOXAN-INDUCED DIABETES (EXPERIMENTAL STUDY)

Abstract

Our study aimed to assess the activity of the blood antioxidant system in the animal model of alloxan-induced diabetes in rats.Diabetes in rats was induced diabetes by a single intraperitoneally injection of freshly prepared 12% aqueous alloxan solution ( 200 mg/kg). The glucose level was measured after 1, 2, 3, 10, 15, and 30 days from alloxan administration. The study included only those rats with blood glucose levels above 250 mg/dl on the 2nd day after alloxan administration.We investigated the activity of antioxidant enzymes, superoxide dismutase (SOD) and catalase (Cat) in the blood serum.The results of the studies show that the activity of enzymatic antioxidant systems in rats' blood sharply increased during the first 3 days after injection of alloxan. That may be due to the compensatory reaction of the body in response to alloxan-induced oxidative stress. Afterwards, the activity of SOD and Cat decreased, which may be due to the utilization of antioxidant proteins in the detoxification process of  ROSs, their glycation and decreased level of the protein synthesis in the body at the intensive hyperglycemia and oxidative stress conditions.