GREEN SYNTHESIS OS SILVER NANOPARTICLES USING METHANOLIC EXTRACTS OF GENTIANA SEPTEMFIDA, ERYSIMUM CONTRACTUM AND CHELIDONIUM MAJUS AND EVALUATION OF ANTIBACTERIAL, ANTIFUNGAL AND CYTOTOXIC ACTIVITIES OF OBTAINED AGNPS
DOI:
https://doi.org/10.52340/jecm.2022.07.36Keywords:
Silver nanoparticles, Eco-friendly, E. contractrum, Ch. Majus, G. septemfidaAbstract
Biosynthesis of metallic nanoparticles is simple, effective, eco-friendly and inexpensive way. It is a non-toxic method. Biosynthesized nanoparticles are primarily biocompatible and can be used in biomedical applications. Silver nanoparticles (AgNPs) are known to have strong antibacterial effects against pathogenic bacteria and have shown antifungal, antiviral activities so they can be helpful not only for fighting against bacteria. In given study silver nanoparticles were biosynthesized using methanolic extracts of Gentiana septemfida, Erysimum contractum and Chelidonium majus. Obtained nanoparticles were evaluated for antibacterial and fungicidal activities against Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus and Candida albicans. Also, their cytotoxic effects were evaluated on human lung carcinoma A-549, colon adenocarcinoma DLD-1, and healthy human skin fibroblasts WS1 cell lines. The results show antibacterial, antifungal and cytotoxic effects at different level for each type. Biosynthesis of silver nanoparticles is simple, cost effective, eco-friendly method and obtained nanoparticles have potential to be used as antibacterial, antifungal and cytotoxic agents.
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Akpinar, I., Unal, M. & Sar, T. Potential antifungal effects of silver nanoparticles (AgNPs) of different sizes against phytopathogenic Fusarium oxysporum f. sp. radicis-lycopersici (FORL) strains. SN Appl. Sci. 3, 506 (2021). https://doi.org/10.1007/s42452-021-04524-5
Alabdallah, Nadiyah M., and Md. Mahadi Hasan. 2021. “Plant-Based Green Synthesis of Silver Nanoparticles and Its Effective Role in Abiotic Stress Tolerance in Crop Plants.” Saudi Journal of Biological Sciences 28 (10): 5631–39. https://doi.org/10.1016/j.sjbs.2021.05.081.
Allawadhi, P., Singh, V., Khurana, A., Khurana, I., Allwadhi, S., Kumar, P., Banothu, A., Thalugula, S., Barani, P.J., Naik, R.R., & Bharani, K.K. (2021). Silver nanoparticle based multifunctional approach for combating COVID-19. Sensors International, 2, 100101 - 100101.
Arif, R. and Uddin, R. (2021), A review on recent developments in the biosynthesis of silver nanoparticles and its biomedical applications. Med Devices Sens, 4: e10158. https://doi.org/10.1002/mds3.10158
Chugh, D., Viswamalya, V.S. & Das, B. Green synthesis of silver nanoparticles with algae and the importance of capping agents in the process. J Genet Eng Biotechnol 19, 126 (2021). https://doi.org/10.1186/s43141-021-00228-w
Cristina Balagna , Sergio Perero, Elena Percivalle, Edoardo Vecchio Nepita, Monica Ferraris; Virucidal effect against coronavirus SARS-CoV-2 of a silver nanocluster/silica composite sputtered coating; Open Ceramics 1 (2020) 100006
D. Beridze , 2019, Phd thesis “Biological and Pharmacognostic study of endemic plant species of Adjara and Adjara-Lazeti”, The Batumi Shota Rustaveli State University
Guilger-Casagrande, Mariana, and Renata de Lima. 2019. “Synthesis of Silver Nanoparticles Mediated by Fungi: A Review.” Frontiers in Bioengineering and Biotechnology 7: 287. https://doi.org/10.3389/fbioe.2019.00287.
Ibrahim, Ezzeldin, Hatem Fouad, Muchen Zhang, Yang Zhang, Wen Qiu, Chengqi Yan, Bin Li, Jianchu Mo, and Jianping Chen. 2019. “Biosynthesis of Silver Nanoparticles Using Endophytic Bacteria and Their Role in Inhibition of Rice Pathogenic Bacteria and Plant Growth Promotion.” RSC Advances 9 (50): 29293–99. https://doi.org/10.1039/C9RA04246F.
Ipe, Deepak S., P. T. Sudheesh Kumar, Robert M. Love, and Stephen M. Hamlet. 2020. “Silver Nanoparticles at Biocompatible Dosage Synergistically Increases Bacterial Susceptibility to Antibiotics.” Frontiers in Microbiology 11: 1074. https://doi.org/10.3389/fmicb.2020.01074.
Jagpreet Singh, Gurleen Kaur, Pawanpreet Kaur, Rajat Bajaj and Mohit Rawat; A REVIEW ON GREEN SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES AND THEIR APPLICATIONS: A GREEN NANOWORLD; World Journal of Pharmacy and Pharmaceutical Sciences, Vol 5, Issue 7, 2016.
Mah TF. Biofilm-specific antibiotic resistance. Future Microbiol. 2012 Sep;7(9):1061-72. doi: 10.2217/fmb.12.76. PMID: 22953707.
Markowska K, Grudniak AM, Wolska KI. Silver nanoparticles as an alternative strategy against bacterial biofilms. Acta Biochim Pol. 2013;60(4):523-30. PMID: 24432308.
Neeraj S. Thakur, Bharat P. Dwivedee, Uttam C. Banerjee and Jayeeta Bhaumik; Bioinspired Synthesis of Silver Nanoparticles: Characterisation, Mechanism and Applications; Silver Nanoparticles for Antibacterial Devices Biocompatibility and Toxicity, Edited by Huiliang Cao; © 2017 by Taylor & Francis Group, LLC
O'Brien J, Wilson I, Orton T, Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur J Biochem. 2000 Sep;267(17):5421-6. doi: 10.1046/j.1432-1327.2000.01606.x. PMID: 10951200.
Rucha Desai, Venu Mankad, Sanjeev K. Gupta, and Prafulla K. Jha; Size Distribution of Silver Nanoparticles: UV-Visible Spectroscopic Assessment; Nanoscience and Nanotechnology Letters Vol. 4, 30–34, 2012
Sharma, D., Misba, L. & Khan, A.U. Antibiotics versus biofilm: an emerging battleground in microbial communities. Antimicrob Resist Infect Control 8, 76 (2019). https://doi.org/10.1186/s13756-019-0533-3
Siddiqi, K.S., Husen, A. & Rao, R.A.K. A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnol 16, 14 (2018). https://doi.org/10.1186/s12951-018-0334-5
Sujata Patra, Sudip Mukherjee, Ayan Kumar Barui, Anirban Ganguly, Bojja Sreedhar, Chitta Ranjan Patra; Green synthesis, characterization of gold and silver nanoparticles and their potential application for cancer therapeutics; Materials Science and Engineering C 53 (2015) 298–309
Vandana B. Parashara, Kulkarni P. Mangesh, Tanwar Sagar, Kiran S.S. Poluri, Kaur Gurmandeep, Kumari Yogita, Behera K. Tusara,Ayinkamiye Clarisse ,Singh K. Sachin ,Wadhwa Sheetu ,Singh Gurvinder ,Kumar Pardeep ,Sachdeva K. Rajesh, “A Review of Reducing Agents in Chemical and Natural Synthesis of Metallic Nanoparticles”, Nanoscience & Nanotechnology-Asia 2021; 11(4) : e270421185388. https://doi.org/10.2174/2210681210999200831100212
Xia ZK, Ma QH, Li SY, Zhang DQ, Cong L, Tian YL, Yang RY. The antifungal effect of silver nanoparticles on Trichosporon asahii. J Microbiol Immunol Infect. 2016 Apr;49(2):182-8. doi: 10.1016/j.jmii.2014.04.013. Epub 2014 May 28. PMID: 24877597.
Zubair Ahmed Ratan, Mohammad Faisal Haidere, Md. Nurunnabi, Sadi Md. Shahriar, A.J. Saleh Ahammad, Youn Young Shim, Martin J.T. Reaney, and Jae Youl Cho ; Green Chemistry Synthesis of Silver Nanoparticles and
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