SELECTION OF STAPHYLOCOCCAL IMMUNOGENES TO OBTAIN HYPERIMMUNE POLYCLONAL SERUM: This work was supported by Shota Rustaveli National Science Foundation of Georgia (SRNSFG), Grant # AR - 18-306

SELECTION OF STAPHYLOCOCCAL IMMUNOGENES TO OBTAIN HYPERIMMUNE POLYCLONAL SERUM

This work was supported by Shota Rustaveli National Science Foundation of Georgia (SRNSFG), Grant # AR - 18-306

Authors

  • SERGO RIGVAVA G. Eliava Institute of Bacteriophages, Microbiology and Virology
  • MERAB NATIDZE G. Eliava Institute of Bacteriophages, Microbiology and Virology
  • LIA GUBELADZE G. Eliava Institute of Bacteriophages, Microbiology and Virology
  • NATIA KARUMIDZE G. Eliava Institute of Bacteriophages, Microbiology and Virology
  • DALI GOGIASHVILI G. Eliava Institute of Bacteriophages, Microbiology and Virology
  • TAMAR TURIASHVILI G. Eliava Institute of Bacteriophages, Microbiology and Virology
  • LALI KAVTARADZE G. Eliava Institute of Bacteriophages, Microbiology and Virology

DOI:

https://doi.org/10.52340/jecm.2022.08.06

Keywords:

staphylococcus, anatoxin, PV-leukocidin, anti-staphylococcal serum

Abstract

Complicated staphylococcal infections: sepsis, meningitis, endocarditis and other requires the development of new generation biological agent with high medicinal property, devoid of adverse events. In this direction, development of microbial cell antigen, anti-hemolytic, anti-leukocidin and anti-hyaluronidase immunoglobulin is promising, which needs staphylococcal immunogene for immunization of producers. Selection of staphylococcal strains was based on the study of morphological, cultural and enzymatic activity, determination of sensitivity to novobiocin and staphylococcal bacteriophage. Among 102 laboratory and clinical staphylococcal strains, following were selected: a) high plasmocoagulating, with hemolytic property, mannitol decomposition and proteolytic property, also St. aureus 24 sensitive to novobiocin and staphylophage and St. epidermidis 6 strains devoid of the mentioned property; b) Thermally inactivated staphylococcal strains, due to their stable characteristics; c) Staphylococcal: alfa-anatoxin, PV-leukocidin and hyaluronidase. Mentioned immunogens were used for priming and immunization of producer-animals (goats). First cycle of immunization has been administered. In the immune serum, the activity of antibodies against the used immunogens is determined by immunoenzymatic analysis, based on „Sandwich-ELISA“ and in passive hemagglutination reaction, using diagnostic test-systems.

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References

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Published

2022-12-07

How to Cite

RIGVAVA, S., NATIDZE, M., GUBELADZE, L., KARUMIDZE, N., GOGIASHVILI, D., TURIASHVILI, T., & KAVTARADZE, L. (2022). SELECTION OF STAPHYLOCOCCAL IMMUNOGENES TO OBTAIN HYPERIMMUNE POLYCLONAL SERUM: This work was supported by Shota Rustaveli National Science Foundation of Georgia (SRNSFG), Grant # AR - 18-306. Experimental and Clinical Medicine Georgia, (8). https://doi.org/10.52340/jecm.2022.08.06

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