SPECTRUM OF ERYTHROCYTE MEMBRANE PROTEIN ABSORPTION IN THE VILLAGES OF SACHKHERE DISTRICT
DOI:
https://doi.org/10.52340/jecm.2022.06.24Keywords:
erythrocytes membrane, UV absorption spectra, protein foldingAbstract
The presented results introduce the absorption intensity of the erythrocytes' membrane proteins of the practically healthy inhabitants of the ethnic homogeneous population of the Sachere region (Georgia).Blood of the practically healthy inhabitants of the villages of the Sachere region - Sareki, Sairhe, and Chorvila, was investigated.The absorption at 230 nm in the erythrocytes' membrane proteins of the inhabitants from Sairhe did not differ from that of the level of the absorption in inhabitants from Chorvilla but was 14% higher in residents from Sareki. It is known that the absorbance at 230 nm is related to the peptide and SS-bonds in the proteins, and its lowering during unfolding proteins and change in their conformation; this can be related to an imbalance of the redox system earlier detected by us in the residents of Sareki in Sairhe.We propose that the intensity of absorption of the erythrocytes' membrane proteins at 230 nm can be considered a risk factor for the development of various chronic diseases, including cancer, associated with oxidative stress.
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Anthis NJ., Clore GM. Sequence-specific determination of protein and peptide concentrations by absorbance at 205 nm. Protein Sci. 2013 Jun; 22(6): 851–858.
Chkhitauri L., Asatiani K., Giorgadze E., Shekiladze E., Sanikidze T. Red Blood Cells membrane proteins in patients with Diabetes Mellitus. Translational and Clinical Medicine-Georgian Medical Journal, 2022; 7(1), 36-39.
Hasts I., Olivia I. Effect on the erythrocytes of the Ca2+/Mg2+-ATP-ase activity. // J. Molecular and Cellular Biochemistry, 1989, Vol. 1, 87-93.
Khetsuriani T., Sanikidze T., Khugashvili R. Alterations of oxidative metabolism at the pregnancy attended with pre-eclampsia. Annals of Biomedical Research and Education, 2004, 4 (12), 81-82.
Marinko JT, Huang H., Penn WD, Capra JA, Schlebach JP, Sanders CR. Folding and Misfolding of Human Membrane Proteins in Health and Disease: From Single Molecules to Cellular Proteostasis. Chem Rev, 2019; 119:5537-5606.
Matoshvili M., Katsitadze A., Sanikidze T., Tophuria D., Richetta A., D'Epiro S. Alterations of redox-status during psoriasis. Georgian Med News, 232, 60-4
Medicherla B, Goldberg AL. Heat shock and oxygen radicals stimulate ubiquitin-dependent degradation mainly of newly synthesized proteins. J Cell Biol. 2008; 182:663–673.
Narayan A. Bhatt at all. Cancer biomarkers - Current perspectives, Indian J Med Res 132, 2010, 129-149.
Pei-Fen Liu P-F, Avramova L, Park Ch. Revisiting absorbance at 230 nm as a protein unfolding probe. Analytical Biochemistry 389 (2009), 165–170.
Reichmann D, Voth W, Jakob U. Maintaining a Healthy Proteome during Oxidative Stress Mol Cell. 2018 January 18; 69(2): 203–213. doi:10.1016/j.molcel.2017.12.021.
Sanikidze T, Chikvaidze E. Role of the free radicals in mechanisms of gallstone formation: An EPR Study Radiation Protection Dosimetry 172 (1-3), 317-324.
Sanikidze TV, Tkhilava NG, Papava MB, Datunashvili IV, Gongadze MT, et al., Role of free nitrogen and oxygen radicals in the pathogenesis of lipopolysaccharide-induced endotoxemia. Bulletin of Experimental Biology and Medicine 141(2), 211-215.
Sharashenidze G, Tsimakuridze M, Chkhikvishvili I, Gabunia T, Gogia N, Ormotsadze G. BAYESIAN MODELLING AND INFERENCE OF MIXTURES OF DISTRIBUTIONS OF BLOOD TOTAL ANTIRADICAL ACTIVITY]. Georgian Med News, 2021 Jun (315):125-128.
Tataradze E., Chabashvili N., Sanikidze T. Physical rehabilitation of stroke patients and redox alterations. Georgian Medical News, 2005 (129):66-69.
Tikaradze E., Bakradze L., Tsimakuridze M., Zedgenidze A., Sanikidze T., Lomadze E., Ormotsadze G. BAYESIAN MODELLING AND INFERENCE OF MIXTURES OF DISTRIBUTIONS OF MICRONUCLEAR BUCCAL CELLS IN THE POPULATION OF SACHKHERE DISTRICT'S VILLAGES. Georgian Med News. 2021 Jul-Aug; (316-317):154-158.
Winterbourn CC, Kettle AJ. Redox reactions and microbial killing in the neutrophil phagosome. Antioxid Redox Signal. 2013 (18):642–660.
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