The Evaluation of False Positive Test Results Possibility of the Derivatives of 1.4 -Benzodiazepines by Immunoassay

Authors

  • Imnadze N.
  • Sivsivadze K.
  • Chapidze N.
  • Murtazashvili T.

Keywords:

Benzodiazepines, Immunoassay

Abstract

Not properly done tests of drugs and toxic agents can cause different problems, and because of that, the main aim of expertize is to select the correct method of analysis. Also, important to create the appropriate flow chart of the analytical stems to use the biological material and time in rational way. Based on, preliminary chemical-toxicological test results of the biological materials the presence of some toxic compounds can be excluded and can be supposed the presence of others. During the planning of our study, the main aim of our work was quantitative evaluation, how many false positive results are confirmed with confirmatory test methods and how reliable it is. Subjects of our study were the derivatives of 1,4-benzodiazepines: Diazepam, Phenazepam and Nitrazepam. During the study was done the extraction of the urine samples by liquid-liquid extraction (alkaline extract) and later for selective separation was used solid phase extraction method. It is notable that solid phase LC18 is very selective and the extraction rate usually is very high.

Downloads

Download data is not yet available.

References

"Diazepam - Drug Usage Statistics". ClinCalc. Retrieved 11 April 2020.

Mandy Alhajj, Aisha Farhana. Enzyme Linked Immunosorbent Assay. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2020 Jan.

D. Shweta, L. Kesharvani, A. K. Gupta, and M. K. Mishra, "Analysis of suspected seized sample of NDPS drugs (benzodiazepines) through GLC and TLC using different solvent system," Indian Journal of Forensic Medicine and Toxicology, vol. 9, no. 1, p. 151, 2015.

17. Thomas O Kohl, Carl A Ascoli. Direct Competitive Enzyme-Linked Immunosorbent Assay (ELISA). Cold Spring Harb Protoc. 2017 Jul 5;

J. Wang, Y. Wang, Y. Pan et al., "Preparation of a broadly specific monoclonal antibody-based indirect competitive ELISA for the detection of benzodiazepines in edible animal

Marieke De Boeck, Giacomo Damilano, Wim Dehaen, Jan Tytgat, Eva Cuypers. Evaluation of 11 ionic liquids as potential extraction solvents for benzodiazepines from whole blood using liquid-liquid microextraction combined with LC-MS/MS. Talant. 2018 Jul 1; 184:369-374.

Published

2021-07-21

How to Cite

Imnadze N., Sivsivadze K., Chapidze N., & Murtazashvili T. (2021). The Evaluation of False Positive Test Results Possibility of the Derivatives of 1.4 -Benzodiazepines by Immunoassay. TSMU COLLECTION OF SCIENTIFIC WORKS, 54. Retrieved from https://journals.4science.ge/index.php/CSW/article/view/421