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Abstract
Georgia is one of the oldest wine regions in the world. Wine industry generates a high amount of by-products, such as shoots, canes, pomace etc., left from grape processing. The interest to these products is growing, as they can be used as a cheap and easily available source of raw materials for the recovery of a lot of bioactive substances for pharmaceutical and cosmetic applications instead of being treated as waste. Polyphenolic compounds are the most valuable of bioactive substance derived from vineyard/wine by-products. The aim of the study was selection of the optimal extraction conditions for TPC (total polyphenol content) from grapevine shoots, to optimize a method for calculation of TPC and evaluation of possible variations of the Folin-Ciocalteu method applied to the shoot raw material and determine TPC in shoots of different samples of Georgian grapevine. Three different samples of raw material shoots were collected in June-July 2019 in Kahketi region (Georgia) - Rkatsiteli (Arkhilo), Rkatsiteli (Alvani) and Saperavi (Arkhilo). The total Polyphenol extraction optimal conditions were selected and quantity was determined expressed as Rutin content. Developed method showed linarity, Precision, Accuracy and Selectivity. Comparative determination of TPC in different samples of grapevine was conducted according to the described method. Study results demonstrated, that shoots of Rkatsiteli (Arkhilo), Rkatsiteli (Alvani) and Saperavi (Arkhilo) contain sum of Polyphenols and the highest value was shown in Rkatsiteli (Arkhilo).
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