How to Cite
Abstract
This study describes the identification and quantification of flavonoids and furostanolic glycosides in plants of the genus Allium: Allium saxatile and Allium ponticum, growing in Georgia. Flavonoids and Furostanol glycosides were identified by thin-layer chromatography (TLC) and quantified by UV-vis spectrophotometry. The furostanol glycosides were visualized on the TLC plate as pink spots after treatment with para-Dimethylaminobenzaldehyde solution, flavonoids were identified as yellow-green spots under 366 nm wavelength, after spraying with a 10 g/L solution of diphenylboric acid aminoethyl ester R in methanol R. Furostanolic glycoside were quantified by UV-Visible spectrophotometry to the amount of at least 0.15% for A. ponticum plant material, and at least 0.6% for A. saxatile. Total ash value for A. ponticum was 4.9%, for A. saxatile_8.1%. During this research, the total flavonoid content (TFC) of both plants crude extract and fractions was determined, and the TFC of crude extract of A. saxatile was 11.07 QE/g, among the fractions, the highest TFC value was found in A.S.F2 with a value of 21.49 QE/g. In case of the crude extract of A. ponticum, the TFC was_13.08 QE/g for the crude extract, and the fraction with the greatest TFC value was found to be A.P.F3 with a value of 16.28 QE/g. The data obtained from this research on the identification and quantification of flavonoid and furostanol glycosides, ash value and moisture content will be used for standardization and quality assessment of raw material.
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