DETERMINATION OF PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY OF GEORGIAN GRAPEVINE LEAVES
DOI:
https://doi.org/10.52340/jecm.2023.04.39Ключевые слова:
Grapevine leaves, Phenolic compounds, Antioxidant activityАннотация
SUMMARY
Vine products (wine, grapes) are rich in beneficial compounds for humans. However, Grapevine cultivation and grape processing generate a large number of by-products, such as grape seed, grape skin and grapevine leaf, most of them can equal or even exceed wine and grapes in terms of the content of biologically active compounds.
Grapevine leaves are a by-product of the vineyard maintenance process. According to the scant information that is currently accessible about Grape leaves, they are rich in organic acids, tannins, procyanidins, anthocyanins, enzymes, vitamins, and carotenoids.
From a scientific and practical standpoint, we considered it necessary to conduct a phytochemical analysis of grapevine leaves. The qualitative composition of phenolic compounds in Rkatsiteli and Saperavi grapevine leaves was examined using the high-performance liquid chromatography-tandem mass spectrometric method (LC-MS-MS) as a result of the performed studies. The obtained results indicate that grape leaves are particularly rich in phenolic acids and flavonoids. The following phenolic compounds have been identified: catechin, epicatechin, quercetin-3-0-glucoside, caffeic acid, trans-resveratrol, etc. (a total of 19 phenolic compounds). The antioxidant activity of the extracts prepared from the raw materials was also studied, which was determined using the DPPH reagent. During the research, a correlation was discovered between the phenolic content of the raw materials and their antioxidant activity. The findings indicate that grapevine leaves contain natural antioxidants that can be used in pharmaceutical, food, and cosmetic production.
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