PROBING ELECTRON TRANSFER AND THERMAL STABILITY OF GLYCATED BY LACTOSE CYTOCHROME C
DOI:
https://doi.org/10.52340/jecm.2025.01.20Keywords:
Cytochrome C, Glycation, Lactoze, cyclic voltammetry, calorimetry, electron transferAbstract
The electron exchange process and thermal stability of the lactose-glycated Cytochrome C (Cyt C) during the 14-day glycation period (T = 25 0C) were observed. The oxidation and reduction reaction rate of Cyt C at a modifier-coated gold electrode, as well as the thermodynamic stability of protein at different stages of the glycation process, were measured using, respectively, cyclic voltammetry and microcalorimetric methods. The obtained experimental results indicate that glycation with lactose leads to a sharp deterioration of the redox activity of Cyt C one week after the beginning of the glycation period. At the same time, the global thermal stability of the protein is maintained for at least two weeks of the glycation period.
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