METHODICAL APPROACHES TO THE DEVELOPMENT OF COMBINED ANTI-ANTRHAX PREPARATION CREATING
Report 2. Creation of anti-anthrax toxin (AAT) hyperimmune serum and study of its antitoxic activity
Abstract
Anthrax toxin (AT) plays a key role in Anthrax pathogenesis and in the formation of acquired immunity. Exotoxin is characterized by lethal, oedematic, protective and immunodepressive activities. B. anthracis exotoxin plays a significant role in the microbe virulence, disease pathogenesis, and causes potential infections induced by vaccinal strains.
The authors derived toxic culture filtrates (TCF) from the following vaccinal strains: STI-1; 55; 34 F2. After the verification of lethal and oedemic activities of the isolated TCF, they were used for hyperimmunization of rabbits. Serum activity was studied by precipitation test in agar jelly. The accumulation of specific antitoxic antibodies in blood was evaluated by the presence and level of precipitation expression lines. The study of the activity of the sera obtained on the 10th day after the final doses of the antigens demonstrated the following: in the sera to be studied, after 48 h, one clear precipitation line was revealed against TCF STI-1, and after 72 h - against TCF 55 and 34 F2.
The data obtained showed that hyperimmunization of the rabbits with vaccinal strains and their toxic culture filtrates was accompanied by the accumulation of the precipitating antibodies in the animal organism after the I hyperimmunization cycle.
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