EFFECT OF E.COLI PHAGE LYSATE VACCINATIONS ON IL-12 ANDIFN-ΓSECRETION AND PARAMETERS OF CANCER GROWTH IN LAB MICE WITH EHRLICH CARCINOMA
Abstract
Anticancer treatment effects of E.coli phage lysate vaccinations (0,25 ml, 1x intraperitoneally, with 5 days interval) have been studied in 2-3 months old 30 lab. mice with Ehrlich carcinoma. The treatment efficacy was estimated by the dynamic of growth of cancer tissue and cancer growth inhibition percent. Immune status was studied by measurement of IL-12 and IFN-γ concentration using ELISA. Investigations have shown that E.coli phage lysate injections delay the cancer growth. Anticancer treatment effect was especially obvious and well expressed in case of 3and 4times vaccinations. During the same period increased secretion of IL-12 and IFN-γ in treated mice compared to the untreated and healthy control was detected. After 5-8 injections the anticancer action of the preparation decreased. Prolonged vaccinations supported progressive cancer growth. Concentration of IL-12 and IFN-γwas decreased as well. The peak of cytokines’ secretion was reached after 3-timesadministration of phagelysate, but after 5-8 vaccinations there were no statistically significant difference between results of experimental and control group animals. Could be concluded that the bacterial preparation - E.coli phage lysate reveal anticancer immunomodulatory properties. For positive treatment results the detection of optimal dosage and regimen of vaccinations is important.
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