GLOBAL DNA METHYLATION LEVELS IN MIGRAINE PATIENTS FROM GEORGIA
DOI:
https://doi.org/10.52340/jecm.2022.08.14Keywords:
Migraine, DNA Methylation, MTHFR, C677TAbstract
Introduction: Migraine is a common, complex neurological disease characterized by the presence of a strong genetic component. Genome-wide association studies (GWAS) have identified gene variants involved in migraine development. In addition, it is known that epigenetic mechanisms, such as DNA methylation, play an important role in inflammation disorders. The aim of our study was to investigate global DNA methylation levels in migraine patients with C677T polymorphism of the MTHFR gene.
Materials and methods: MTHFR C677T genotyping analysis (36 patients and 32 controls) was performed using TaqMan technology (Thermo Scientific, USA). Global DNA methylation was determined by the quantitative analysis of 5-methylcytosine (Zymo Research, Germany).
Results: In individuals with TT genotypes, the percentage of methylated cytosine (5mC) was significantly lower in the migraine group compared to the control group (P=0.001).
Conclusions: Our results suggest that epigenetic approaches can be used to study the migraine pathogenesis. In addition, reduced methylation levels can be ameliorated by the dietary supplementation with vitamins B6, B9, B12, which may be a strategy for migraine prevention and management in patients with MTHFR C677T polymorphism.
Downloads
References
Headache Classification Committee of the International Headache Society (IHS). The International Classification of Headache Disorders, 3rd edition (beta version). Cephalalgia. 2013 Jul;33(9):629-808. doi: 10.1177/0333102413485658. PMID: 23771276.
Wessman M, Terwindt GM, Kaunisto MA, Palotie A, Ophoff RA. Migraine: a complex genetic disorder. Lancet Neurol. 2007 Jun;6(6):521-32. doi: 10.1016/S1474-4422(07)70126-6. PMID: 17509487.
MacGregor EA. Classification of perimenstrual headache: clinical relevance. Curr Pain Headache Rep. 2012 Oct;16(5):452-60. doi: 10.1007/s11916-012-0282-y. PMID: 22653664.
Pietrobon D, Striessnig J. Neurobiology of migraine. Nat Rev Neurosci. 2003 May;4(5):386-98. doi: 10.1038/nrn1102. PMID: 12728266.
Tsang BL, Devine OJ, Cordero AM, Marchetta CM, Mulinare J, Mersereau P, Guo J, Qi YP, Berry RJ, Rosenthal J, Crider KS, Hamner HC. Assessing the association between the methylenetetrahydrofolate reductase (MTHFR) 677C>T polymorphism and blood folate concentrations: a systematic review and meta-analysis of trials and observational studies. Am J Clin Nutr. 2015 Jun;101(6):1286-94. doi: 10.3945/ajcn.114.099994. Epub 2015 Mar 18. PMID: 25788000.
Crider KS, Yang TP, Berry RJ, Bailey LB. Folate and DNA methylation: a review of molecular mechanisms and the evidence for folate's role. Adv Nutr. 2012 Jan;3(1):21-38. doi: 10.3945/an.111.000992. Epub 2012 Jan 5. PMID: 22332098; PMCID: PMC3262611.
Ueland PM, Hustad S, Schneede J, Refsum H, Vollset SE. Biological and clinical implications of the MTHFR C677T polymorphism. Trends Pharmacol Sci. 2001 Apr;22(4):195-201. doi: 10.1016/s0165-6147(00)01675-8. PMID: 11282420.
Abzianidze E, Kvaratskhelia E, Tkemaladze T, Kankava K, Gurtskaia G, Tsagareli M. Epigenetic regulation of acute inflammatory pain. Georgian Med News. 2014 Oct;(235):78-81. PMID: 25416223.
Costello JF. DNA methylation in brain development and gliomagenesis. Front Biosci. 2003 Jan 1;8:s175-84. doi: 10.2741/1027. PMID: 12456310.
Eising E, A Datson N, van den Maagdenberg AM, Ferrari MD. Epigenetic mechanisms in migraine: a promising avenue? BMC Med. 2013 Feb 4;11:26. doi: 10.1186/1741-7015-11-26. PMID: 23379668; PMCID: PMC3584973.
Steiner TJ, Stovner LJ, Birbeck GL. Migraine: the seventh disabler. J Headache Pain. 2013 Jan 10;14(1):1. doi: 10.1186/1129-2377-14-1. PMID: 23566305; PMCID: PMC3606966.
Younger DS. Epidemiology of Migraine. Neurol Clin. 2016 Nov;34(4):849-861. doi: 10.1016/j.ncl.2016.06.011. PMID: 27719997.
Downloads
Published
How to Cite
Issue
Section
