GHRELIN LEVEL CHANGES IN RATS WITH TYPE 2 DIABETES MELLITUS
DOI:
https://doi.org/10.52340/jecm.2022.06.30Keywords:
T2DM, Ghrelin, Glucose levels, STZAbstract
Purpose: The aim of our research was to identify the ghrelin concentration in experimental animals before and after low doses of Streptozotoccin injection.
Materials and methods: Experiments were performed on male Wistar rats. Animals were divided into two groups; control and experimental group. In the control group animals had normal weight. Animals from the experimental group were divided into three subgroups, based on the rats weight: normal weight group (Group I), overweight group (Group II) and obesity group (Group III). Glucose levels were tested 4 weeks after multiple low doses of STZ injection. All rats with fasting blood glucose concentrations greater than 14 mmol/l were considered to be diabetic and were selected for further research. In all rats (control and experimental group), ghrelin levels were tested before and after multiple low doses of STZ injection.
Results: Ghrelin levels were higher in normal weight rats than in obese and overweight subjects. In the experimental group, after multiple low doses of Streptozotocin injection (30 mg/kg- twice) Ghrelin levels increased proportionately in all three groups.
Conclusion: At the beginning of our experiment, in subjects without T2DM, ghrelin levels were higher in normal weight rats than in obese and overweight subjects, but after multiple low doses of streptozotocin injection (30 mg/kg- twice), ghrelin levels increased proportionately in all three groups. As is known from recent research, ghrelin inhibits insulin secretion, and in the situation where there is an already high insulin resistance, in type 2 diabetes, ghrelin levels increase with diabetes compared to a control group, and it potentially decreases the effectiveness of endogenous insulin. It has also been observed that an increase of ghrelin is accompanied by increased glucose levels. Results of the experiment confirms ghrelin’s suppressive effect on insulin secretion.
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Kojima M, Hosoda H, Date Y, et al. Ghrelin, a Novel Growth Hormone-Releasing Acylated Peptide, Is Synthesized in a Distinct Endocrine Cell Type in the Gastrointestinal Tracts of Rats and Humans. Nature 1999; 402:656.
Takaya K, Ariyasu H, Kanamoto N, et al. Ghrelin strongly stimulates growth hormone release in humans. J Clin Endocrinol Metab 2000; 85:4908.
Benso A, Casanueva FF, Ghigo E, Granata A (eds): The Ghrelin System. Endocr Dev. Basel, Karger, 2013, vol 25, pp 135–143 (DOI: 10.1159/000346064)
Cummings DE, Purnell JQ, Frayo RS, Schmidova K, Wisse BE, Weigle DS: A preprandial rise in plasma ghrelin levels suggests a role in meal initiation in humans. Diabetes 2001; 50:1714-1719.
Mundinger TO, Cummings DE, Taborsky GJ Jr. Direct stimulation of ghrelin secretion by sympathetic nerves. Endocrinology 2006; 147:2893.
Zhang M, Lv XY, Li J, Xu ZG, Chen L. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Experimental Diabetes Research 2008; 2008: 1–9.
Liu Z, Li W, Li X, Zhang M, Chen L, Zheng YN, et al. Antidiabetic effects of malonyl ginsenosides from Panax ginseng on type 2 diabetic rats induced by high-fat diet and streptozotocin. Journal of Ethno- pharmacology 2013; 145: 233–40. doi: 10.1016/j.jep.2012.10.058 PMID: 23147499
Ibrahim Abdalla M. M. (2015). Ghrelin - Physiological Functions and Regulation. European endocrinology, 11(2), 90-95. https://doi.org/10.17925/EE.2015.11.02.90
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