Study of the inhibitory effect of Silver Nanoparticles on Extensively Drug-resistant Mycobacterium Tuberculosis (TB) strains

Study of the inhibitory effect of Silver Nanoparticles on Extensively Drug-resistant Mycobacterium Tuberculosis (TB) strains

Authors

DOI:

https://doi.org/10.52340/spectri.2024.09.01.12

Keywords:

Silver Nanoparticles, pre-extensively drug-resistant Tuberculosis, Mycobacterium Tuberculosis

Abstract

Introduction: One of crucial focus within phthisiology is the exploration and analyses of substances capable of overcoming resistance to anti-TB medications in drug-resistant mycobacterium TB strains. While substantial experimental data confirm the bactericidal effect of silver nanoparticles (AgNPs) on various drug-resistant strains, there remains the notable gap in understanding their action against Mycobacterium tuberculosis(MTB), particularly in drug-resistant cases. The inhibitory efficacy of AgNPs on MTB strains causing pre-XDR-TB has remained largely unexplored. Our study was specifically aimed at investigating this aspect. Methods: In this study, 20-nanometer AgNPs were used. To create the nanoparticle suspension, a high-frequency ultrasound homogenizer disruptor disintegrator (Ultrasonic Processor FS-1800N) was utilized.  The in vitro experiment was conducted using 35 cultures of rifampicin and fluoroquinolone-resistant Mycobacterium TB (pre-XDR-MTB) obtained from participants’ sputum samples with tuberculosis. The experiment was conducted in a liquid media using BACTEC MGIT 960 system.                            Results: The conducted study demonstrated that adding 0.25%, 0.5% and 1.0% AgNPs suspensions to pre-XDR-MTB strains was completely ineffective; The growth of preXDR-MTB strains was inhibited, with minimal inhibitory effects observed when using a 2.5% AgNPs solution. Complete inhibition and a bactericidal effect were noted with the addition of 5% and 10% AgNPs solutions.      Conclusion: The experimental study confirmed the inhibitory effect of AgNPs on preXDR-MTB  strains. These results provide a solid foundation for further research to investigate the safety of using AgNPs and their potential to restore the efficacy of anti-TB medications.

Downloads

Download data is not yet available.

Author Biographies

Nino Kiria, Tbilisi State University

MD, PhD student

Teona Avaliani, Tbilisi State University

MD, PhD student

Nino Bablishvili, National Center for Tuberculosis and Lung Diseases

PhD

Giorgi Phichkhaia, Tbilisi State University

Professor

Nana Kiria, David Agmashenebeli University of Georgia

Professor

References

WHO. Global antimicrobial resistance and use surveillance system (‎GLASS)‎ report: 2022: 1-4.

Patel J, Harant A, Fernandes G, Mwamelo AJ, Hein W, Dekker D. Measuring the global response to antimicrobial resistance, 2002-21: a systematic governance analysis of 114 countries. The Lancet, Infectious diseases. 2023; 23 (6):706-718.

WHO. Consolidated guidelines on tuberculosis: module 4: treatment: drug-resistant tuberculosis treatment. 2022.

WHO. Global tuberculosis report. 2023.

Uba Bo, Okoye EL, Anyichie JC, Dokubo CU, Uqwuojj ET. Measuring the global response to antimicrobial resistance.Journal of Advances in microbiology.2024; (3):65-78.

Sadhu S, Karmakar T, Chatterjee AG, Kumari U, Mondal P, Sarka S, Sur T, Tarafdar S. International Journal of Experimental Research and Review. 2022 (29):67-72.

F. Andrade F. Nanotechnology and pulmonary delivery to overcome resistance in infectious diseases. Advanced Drug Delivery Reviews.2013 (l65): 1816-1827.

Parashar s, Sharma MK, Garg Ch, Garg M. Green Synthesized Silver Nanoparticles as Silver Lining in Antimicrobial Resistance: A Review. Current Drug Delivary. 2022 (19):170-181.

Farooq U, Ahmad T, Khan A,Sarwar R, Shafig J, Raza Y, Ahmed A, Ullah S, Rehman NU, Al-Harrasi A. Rifampicin conjugated silver nanoparticles: a new arena for development of antibiofilm potential against methicillin resistant Staphylociccus aureus and Klebsiella pneumoniae. International Journal Nanomedicine. 2019 (14):3983-3993.

Zaharov AV, Khokhlov AL, Ergeshov AE. Silver nanoparticles in the solution of the problem of drug resistance in mycobacterium tuberculosis. Archive of Internal Medicine. 2017 (3):188-199.

Downloads

Published

2024-07-10

How to Cite

Kiria, N., Avaliani, T., Bablishvili, N., Phichkhaia, G., & Kiria, N. (2024). Study of the inhibitory effect of Silver Nanoparticles on Extensively Drug-resistant Mycobacterium Tuberculosis (TB) strains. Scientific Journal „Spectri“, 9(1). https://doi.org/10.52340/spectri.2024.09.01.12

Issue

Section

Articles
Loading...