CHEMICAL COMPOSITION OF HYDROLYZATE OF AQUEOUS EXTRACT OF SALVIA GAREDJI
DOI:
https://doi.org/10.52340/jecm.2026.01.03Keywords:
Salvia garedji, phenolic acids, depsides, flavonoids, phenolic diterpens, HPLC-MSAbstract
The aqueous extract of the aerial parts of the Caucasian endemic plant Salvia garedji Troitzk. (family - Lamiaceae Lindl.) is characterized by an abundant content of phenolic compounds (49.8% tannin by permanganometry). To determine the nature of polyphenols, acidic hydrolysis of the aqueous extract was performed. HPLC-MS analysis of the hydrolysate revealed the presence of phenolic acids, their derivatives, terpene phenols, and flavonoids (a total of 32 substances). 13 of them - carnosol, carnosic acid, yunnaneic acid F, sagerinic acid, salvianolic acid A, salvianolic acid A isomer, luteolin-7-0-glucuronide, apigenin-7-0-glucuronide, rosmarinic acid, methyl gallate, cirsimaritin, epigallocatechin, danshensu (salvianic acid A) - were previously identified in the native aqueous extract. 19 substances - rosmanol, salvianolic acid F, rosmaridiphenol, caffeoyl threonic acid, p-hydroxybenzoyl glucose, caftaric acid, miltirone, ethyl rosmarinate, yunnaneic acid E, yunnaneic acid D, eriodictyol-di-glucoside, rosmarinic acid isomer, yunnaneic acid H, cirsilineol, feruloyl tartrate (fertaric acid), 6-hydroxy-luteolin-7-O-glucuronide, chrysin-7-glucuronide, 5-hydroxy-6,7,3′,4′-tetramethoxyflavone, 3-O-feruloylquinic acid - are products of hydrolysis. The absence of gallic and ellagic acid residues in the hydrolysate indicates that the extract does not contain hydrolysable tannins. Therefore, the result (49.8 %) obtained by the permanganometric titration method for the quantitative determination of tannin substances in plant raw materials given in the State Pharmacopoeia is actually due to the oxidation of polyphenols of another groups. In various in vitro experiments, the aqueous extract showed high antioxidant activity.
Downloads
References
Sagareishvili T. G., Getia M. Z. Chemical comрosition of Salvia garedji, an edemic рlant of the Сaucasus. Chem. Nat. Compd., 60, 4, 2024, 766 - 767.
Государственная фармакопея Российской федерации. Определение содержания дубильных вешеств в лекарственном растительном сырье и лекарственных растительных препаратах. ХIV.Москва, II, 2018, 2365-69
Amaro-Luis J. M. Abietane diterpenoids from Salvia rubescens ssp. truxillensis. Pharm. Acta Helv. 72, 1997, 233-8
Afonso A. F., Pereira O. R., Fernandes A. et al. Phytochemical composition and bioactive effects of Salvia africana, Salvia officinalis ‘Icterina’ and Salvia mexicana Aqueous Extracts. Molecules, 24, 2019, 4327.
Mena P., Cirlini M., Tassotti M., Herrlinger K. A. et al. Phytochemical profiling of flavonoids, phenolic acids, terpenoids, and volatile fraction of a rosemary (Rosmarinus officinalis L.) extract. Molecules, 21, 2016, 1576.
Krzyzanowska-Kowalczyk J., Recio L., Moldoch J., Ludwiczuk A., et al. Novel phenolic constituents of Pulmonaria officinalis L. LC-MS/MS comparison of spring and autumn metabolite profiles. Molecules, 23, 2018, 2277.
Cvetkovikj C., Stefkov G., Acevska J., Petreska Stanoeva J., Karapandzova M., Stefova M., Dimitrovska A., Kulevanova S. Polyphenolic characterization and chromatographic methods for fast assessment of culinary Salvia species from South East Europe. J. Chromatogr. A, 1282, 2013, 38 –45.
Carocho M., Barros L., Barreira J.C.M., Calhelha R.C., Sokovic M., Fernandez-Ruiz V., Buelga C.S. et al. Basil as functional and preserving ingredient in “Serra da Estrela” cheese. Food Chemistry, 207, 2016, 51-59.
Keramat M., Golmakani M. T. Effects of rosmarinic acid esters on the oxidation kinetic of organogel and emulsion gel. Food Chemistry: X, 22, 2024, 101343.
Tanaka T., Nishimura A., Kouno I. et al. Magnesium and ammoniumpotassium lithospermates B, the active principles having a preventive effect from Salvia miltiorrhiza. Chem. Pharm. Bull. 45 (10), 1997, 1596-1600.
Ribeiro A., Caleja C., Barros L. et al. Rosemary extracts in functional foods: Extraction, chemical characterization and incorporation of free and microencapsulated forms in cottage cheese. Food Funct. 2016, 7, 2185–2196.
Afonso A. F., Pereira O. R., Válega M. et al. Metabolites and Biological Activities of Thymus zygis, Thymus pulegioides, and Thymus fragrantissimus Grown under Organic Cultivation. Molecules, 23, 2018, 2514.
Xu Y. Y., Wan R. Z., Lin Y. P., Yang L., Chen Y., Liu C. X. Recent advance on research and application of Salvia miltiorrhiza. Asian J. of Pharmacodynamics and Pharmacokinetics, 7, 2, 2007, 99–130.
Kammerer D. et al. Polyphenol screening of pomace from red and white grape varieties (Vitis vinifera L.) by HPLC-DAD-MS/MS. Journal of Agricultural and Food Chemistry. J. Agric. Food Chem., 52, 14, 2004, 4360–4367.
Zimmermann B. F., Walch S. G., Tinzoh L. N., Stuhlinger W., Lachenmeier D. W. Rapid UHPLC determination of polyphenols in aqueous infusions of Salvia officinalis L. (sage tea). J. Chromat. B, 879, 24, 2011, 2459 – 2464.
Rojsanga P., Schwaiger S. et al. Determination of phytochemical contents in extracts from different growth stages of Oroxylum indicum Fruits using HPLC-DAD and QAMS methods. Molecules, 28, 2023, 6837.
Sagareishvili T. G., Alaniya M. D. Phenolic compounds from the tubular flowers of Leucanthemum vulgare. Chem. Nat. Compd., 27, 1991, 512.
Moradkhani S., Kobarfard F., Ayatollahi S.A.M. Phytochemical investigations on chemical constituents of Achillea tenuifolia Lam. Iran. J. Pharm Res. 13, 3, 2014, 1049 – 1054.
Afonso A. F., Pereira O. R., Fernandes A.S.F., Calhelha R. C. et al. The health-benefits and phytochemical profile of Salvia apiana and Salvia farinacea var.Victoria Blue decoctions. Antioxidants, 8, 2019, 241.
Grenier A., Legault J., Pichette A., Jean L., et al. Antioxidant, anti-inflammatory, and anti-aging potential of a Kalmia angustifolia extract and identification of some major compounds. Antioxidants, 10, 9, 2021, 1373.
Андреева Л. И., Андреева Л. И., Кожемякин Я. А., Кушкин А. А. Модификация метода определения перекисей липидов в тесте с тиобарбитуровой кислотой. Лабораторное дело, 11, 1988, 41-43.
Downloads
Published
How to Cite
Issue
Section
