PHARMACOLOGICAL AND BIOCHEMICAL STUDIES OF VIPER VENOM (MACROVIPERA LEBETINA OBTUSA LINNAEUS, 1758) DEPENDING ON SHELF LIFE
DOI:
https://doi.org/10.52340/jecm.2024.04.20Keywords:
viper venom, Macrovipera lebetina obtusa Linnaeus, enzyme activity, phospholipase A2, L-amino acid oxidases, proteolytic activityAbstract
The purpose of this work is to study the dynamics of changes in the activity of phospholipase A2, proteolytic activity (PA), and activity of L-amino acid oxidase (AO) of venom samples, venom of viper (Macrovipera lebetina obtusa Linnaeus, 1758) with different storage periods, collected over a while since 1989 - 2015 Enzyme activity in samples of Transcaucasian viper venom was determined by titrometric method.
Phospholipase A2 activity during storage since 1989 decreased by 11.5 IU/mg compared to the venom sample collected in 2015. In all likelihood, as a result of biochemical changes, there is a decrease in enzyme activity from 100% to 72.61, 77.38, 82.38, and 95.24%, respectively, which must be taken into account when producing and storing preparations based on snake venom.
In venom samples collected in 2015, the L-amino acid oxidase activity is 0.30 IU/mg. In samples of viper venom collected in 2015, the content of AO is higher by 3.33, 2.73, 2.0, and 1.15 times than in samples collected in 1989, 1991, 1993, and 2010, respectively. A decrease in the enzymatic activity of viper venom was revealed during long-term storage from 1989 to 2015.
It has been experimentally established that during long-term storage of viper venom samples from the storage period from 1989, 1991, 1993 to 2010, there is a significant decrease in the activity of the phospholipase A2 enzyme, which corresponds to 72.61, 77.38, 82.38, 95.24%, respectively.
It was revealed that in samples of viper venom collected in 2015, the PA content is higher by 2.73, 2.0, 1.46, and 1.71 times than in venom samples collected in 1989, 1991, 1993, and 2010, respectively.
The level of L-amino acid oxidase (AO) activity was determined. In venom samples collected in 2015, the L-amino acid oxidase activity is 0.30 IU/mg. In samples of viper venom collected in 2015, the content of AO is higher by 3.33, 2.73, 2.0, and 1.15 times than in samples collected in 1989, 1991, 1993, and 2010, respectively.
The results of experimental data can be used in identifying, standardizing, and determining the pharmacological activity of viper venom.
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