Как цитировать
Аннотация
A sensitive, accurate and efficient liquid chromatographicmethod has been developed for determination of nitrofuranresidues in a honey sample;It was found that the detection minimum for all four nitro-furan group substances is 0.03 ìg / ml, the specific wave-length is 376 nm, the specificity coefficient of variation forboth on one working day and one working week is <2%,accuracy for solutions of three different concentrations is<2 %, The accuracy of the method for all four test substanc-es is <2%, indicating the relevance of the method specificityto the criteria set for the analytical methods;The caliber graph for nitrofuran group is linear in therange 0.03 ìg / ml - 1 ìg / ml - 1 - 5 ìg / ml and is acceptable aslong as the correlation coefficient in all cases is> 0.999 (>0.99);Sample preparation is satisfactory as the obtained sam-pling coefficient is> 0.6.Thus, as a result of validation of the liquid chromato-graphic method developed for the quantitative determina-tion of nitrofuran residues in the honey sample, the methodwas fully complied with the requirements of the Governmentof Georgia according to the following validation characteris-tics: detection minimum, specificity, accuracy, correctnessand linearity. (#499, Guidance for Industry BioanalyticalMethod Validation U.S. Department of Health and HumanServices Food and Drug Administration Center for DrugEvaluation and Research (CDER) Center for Veterinary Med-icine (CVM) May 2001)