IMPACT OF HYPERGLYCEMIA ON ERYTHROCYTE'S MEMBRANE BAND 3 PROTEIN (BP3)

LELA CHKHITAURI; EKA SHEKILADZE; MAIA MANTSKAVA; KETEVAN ASATIANI; ELENE GIORGADZE; IRINA KAPETIVADZE; TAMAR SANIKIDZE

doi:10.52340/jecm.2023.02.01

IMPACT OF HYPERGLYCEMIA ON ERYTHROCYTE'S MEMBRANE BAND 3 PROTEIN (BP3)

Авторы

LELA CHKHITAURI Tbilisi State Medical University
EKA SHEKILADZE Tbilisi State Medical University
MAIA MANTSKAVA Tbilisi State Medical University
KETEVAN ASATIANI Tbilisi State University
ELENE GIORGADZE Tbilisi State University
IRINA KAPETIVADZE Tbilisi State Medical University
TAMAR SANIKIDZE Tbilisi State Medical University

DOI:

https://doi.org/10.52340/jecm.2023.02.01

Ключевые слова:

bend 3 protein, erythrocytes, hyperglycemia

Аннотация

To prevent T2DM and its chronic complications early diagnostic of carbohydrates metabolism disorders is necessary. We investigated the erythrocytes membrane proteins in groups of patients with insulin resistance, prediabetes, and T2DM. Blood was obtained from healthy volunteers and patients with T2DM, prediabetes, and IR. Band 3 protein (B3p) content in isolated erythrocyte membranes was determined with the analytical electrophoresis method. АNOVA was used for the comparative analysis of the data. In patients with disorders of carbohydrate metabolism the expression of B3p (90-100kDa) was reduced in comparison to the control level and appeared as the oligomerized form of B3p (180kDa). Therefore, it seems that in the process of modification of erythrocyte membranes of patients with disorders of carbohydrate metabolism participate both, hyperglycemia and oxidative stress conditions associated with diabetes.

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Библиографические ссылки

Haffner S.M., Mykkanen L., Festa A. et al. Insulin-resistant prediabetic subjects have more atherogenic risk factors than insulin-sensitive prediabetic subjects: implication for preventing coronary heart disease during the prediabetic state. Circulation 2000; 101: 975–980.

American Diabetes Association. Prevention or Delay of Type 2 Diabetes: Standards of Medical Care in Diabetes. 2018. Diabetes Care. 2018; 41(Supplement 1):51-54.

Hasts I., Olivia I., Effect on the erythrocytes of the Ca2+/Mg2+-ATP-ase activity. // J. Molecular and Cellular Biochemistry, 1989; 1: 87-93.

Laemmli V. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. J. Nature, 1970;227:680-685

Reithmeier, R.A.; Casey, J.R.; Kalli, A.C.; Sansom, M.S.; Alguel, Y.; Iwata, S. Band 3, the human red cell chloride/bicarbonate anion exchanger (AE1, SLC4A1), in a structural context. Biochim. Biophys. Acta 2016,1858, 1507–1532

Oliveira S., Saldanha C., An overview about erythrocyte membrane. Clin Hemorheol Microcirc. 2010;44(1):63-74.

Pantaleo A., Ferru E, Carta F, Valente E, Pippia P, Turrini F. Effect of heterozygous beta thalassemia on the phosphorylative response to Plasmodium falciparum infection. J Proteomics. 2012;5,76:251-8.

Ferru E, Giger K, Pantaleo A, Campanella E, Grey J, Ritchie K, Vono R, Turrini F, Low PS. Regulation of membrane-cytoskeletal interactions by tyrosine phosphorylation of erythrocyte band 3. Blood. 2011; 2,117(22):5998-6006.

Lela Chkhitauri, Tamar Sanikidze, Elene Giorgadze, Ketevan Asatiani, Nana Kipiani, Nana Momtselidze, and Maka Mantskava. Comprehensive study of the rheological status and intensity of oxidative stress during the progression of type 2 diabetes mellitus to prevent its complications. Clinical Hemorheology and Microcirculation. 2023; 83:69–79.