EXPRESSION OF CHROMOGRANIN (CGA) IN RAT PANCREAS DURING ALLOXAN DIABETES
DOI:
https://doi.org/10.52340/jecm.2024.02.09Keywords:
Chromogranin, CgA, pancreas, Alloxan, Diabetes, RatAbstract
Using morphological research methods, the pancreas of rats with alloxan diabetes was studied. In the study, special attention was paid to studying the expression of chromogranin CgA. It is known that CgA-glycoprotein belongs to the group of granins. It is abundant in pancreatic neurons, neuroendocrine and endocrine cells and plays an important role in the generation of secretor granules. CgA-marker specially designed for these cells was used to determine the level of their expression, while silver staining was performed for better visualization of NE cells. Studies have shown that with moderate severity of Alloxan diabetes, a strong expression of CgA was observed in the NE of the remaining endocrine and also in the cells of the intralobular ducts of the pancreas. Based on the data obtained, it can be assumed that in Alloxan diabetes there is an increase in the intracellular function of NE cells, which affects the maturation of granules, secretion and intracellular storage of granules. Positive expression in intralobular epithelial cells of the pancreatic ducts suggests that CgA may also be an important marker for identifying pancreatic stem cells.
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