TY - JOUR AU - Gotsiridze , D. AU - Baramidze , K. AU - Chikviladze , T. AU - Otarashvili , T. AU - Ioramashvili , H. PY - 2023/01/31 Y2 - 2024/03/29 TI - DEVELOPMENT AND VALIDATION OFLIQUID CHROMATOGRAPHIC METHODFOR THE DETERMINATIONNITROFURAN RESIDUES IN HONEY JF - Collection of Scientific Works of Tbilisi State Medical University JA - CSW VL - 55 IS - SE - Articles DO - UR - https://journals.4science.ge/index.php/CSW/article/view/1423 SP - AB - <p>A sensitive, accurate and efficient liquid chromatographicmethod has been developed for determination of nitrofuranresidues in a honey sample;It was found that the detection minimum for all four nitro-furan group substances is 0.03 ìg / ml, the specific wave-length is 376 nm, the specificity coefficient of variation forboth on one working day and one working week is &lt;2%,accuracy for solutions of three different concentrations is&lt;2 %, The accuracy of the method for all four test substanc-es is &lt;2%, indicating the relevance of the method specificityto the criteria set for the analytical methods;The caliber graph for nitrofuran group is linear in therange 0.03 ìg / ml - 1 ìg / ml - 1 - 5 ìg / ml and is acceptable aslong as the correlation coefficient in all cases is&gt; 0.999 (&gt;0.99);Sample preparation is satisfactory as the obtained sam-pling coefficient is&gt; 0.6.Thus, as a result of validation of the liquid chromato-graphic method developed for the quantitative determina-tion of nitrofuran residues in the honey sample, the methodwas fully complied with the requirements of the Governmentof Georgia according to the following validation characteris-tics: detection minimum, specificity, accuracy, correctnessand linearity. (#499, Guidance for Industry BioanalyticalMethod Validation U.S. Department of Health and HumanServices Food and Drug Administration Center for DrugEvaluation and Research (CDER) Center for Veterinary Med-icine (CVM) May 2001)</p> ER -